Collaborators:
Gabriella Muinde
Camille Carr
Madison
Abstract:
For many years up north salt has been spilled on the highways and streets before and after a snowstorm to try and avoid to much ice from being formed on the roads. But there has been some people that say that this salt may affect the growth of vegetation along the roads and plant that are in nearby streams. So for this lab were very going to test how radish seeds would be effected by this salt. With the six different petri dishes we added different amounts of salt to each so we could see how the different amounts of salts would affect the results. After letting it sit for a couple of days we examined all of the different petri dishes and came to conclusions on whether or not the salt is affect the growth of vegetation.
Problem:
How does the concentration of salt water effect radish seed germination?
Hypothesis:
I believe that the more salt that is added onto the streets the more affects it will have on the vegetation.
Parts of the Experiment:
Control group - The group with no salt (1st petri dish)
Experimental groups- petri dish 2-5 that had the different amounts of salt in them
Independent Variable- dose
Dependant Variable- response
Controlled variables- control group
Materials:
- 6 petri dishes
- 6 seeds
- 12 napkins
- Concentrated salt water solution
- water
- graduated cylinder
Procedure:
1. Use the graduated cylinders and test tubes to prepare the various concentrations as shown in the graph.
2. Label all petri dishes with your group number, the dish number, and a percent concentration of chemical:
a. Dish #1: 0%
b. Dish #2: 6.25%
c. Dish #3: 12.5%
d. Dish #4: 25%
e. Dish #5: 50%
f. Dish #6: 100%
3. Put two napkins together and cut them so that they fit into the petri dish.
4. Put on the safety goggles and latex gloves. Carefully pour the chemical solutions onto the napkins, make sure to match the numbers and concentration percentages of the dish.
5. Count out 10 seeds. Carefully place the seeds on the moist napkins in the petri dish.
6. Repeat steps 3-5 for the other dishes.
7. Place the seed dishes in a stack, lying flat with the seeds up. Put the seeds in the spot designated by your teacher.
Collecting Data and Plotting Results:
1. Remove the lid of the control dish. Count the number of seeds that germinated. Calculate the percentage of seeds that germinated and record in Table 2.
2. Measure the length of the radical for each of the germinating lettuce seeds to the nearest mm. Look carefully at each sprout to make sure you are measuring just the root, not the shoot as well.
3. Repeat steps 3-5 for each petri dish.
4. Make a line graph from the data collected to show a dose-response curve.
Data:
Gabriella Muinde
Camille Carr
Madison
Abstract:
For many years up north salt has been spilled on the highways and streets before and after a snowstorm to try and avoid to much ice from being formed on the roads. But there has been some people that say that this salt may affect the growth of vegetation along the roads and plant that are in nearby streams. So for this lab were very going to test how radish seeds would be effected by this salt. With the six different petri dishes we added different amounts of salt to each so we could see how the different amounts of salts would affect the results. After letting it sit for a couple of days we examined all of the different petri dishes and came to conclusions on whether or not the salt is affect the growth of vegetation.
Problem:
How does the concentration of salt water effect radish seed germination?
Hypothesis:
I believe that the more salt that is added onto the streets the more affects it will have on the vegetation.
Parts of the Experiment:
Control group - The group with no salt (1st petri dish)
Experimental groups- petri dish 2-5 that had the different amounts of salt in them
Independent Variable- dose
Dependant Variable- response
Controlled variables- control group
Materials:
- 6 petri dishes
- 6 seeds
- 12 napkins
- Concentrated salt water solution
- water
- graduated cylinder
Procedure:
1. Use the graduated cylinders and test tubes to prepare the various concentrations as shown in the graph.
2. Label all petri dishes with your group number, the dish number, and a percent concentration of chemical:
a. Dish #1: 0%
b. Dish #2: 6.25%
c. Dish #3: 12.5%
d. Dish #4: 25%
e. Dish #5: 50%
f. Dish #6: 100%
3. Put two napkins together and cut them so that they fit into the petri dish.
4. Put on the safety goggles and latex gloves. Carefully pour the chemical solutions onto the napkins, make sure to match the numbers and concentration percentages of the dish.
5. Count out 10 seeds. Carefully place the seeds on the moist napkins in the petri dish.
6. Repeat steps 3-5 for the other dishes.
7. Place the seed dishes in a stack, lying flat with the seeds up. Put the seeds in the spot designated by your teacher.
Collecting Data and Plotting Results:
1. Remove the lid of the control dish. Count the number of seeds that germinated. Calculate the percentage of seeds that germinated and record in Table 2.
2. Measure the length of the radical for each of the germinating lettuce seeds to the nearest mm. Look carefully at each sprout to make sure you are measuring just the root, not the shoot as well.
3. Repeat steps 3-5 for each petri dish.
4. Make a line graph from the data collected to show a dose-response curve.
Data:
Analysis and Conclusion:
1. Based on the graph that my group collected I would say that the LD 50 for this graph would be 80%. This would be true because in our table at 50% concentration of salt 80% of the seeds were germinated. Then at 100% concentration of salt 0% seeds were germinated. Meaning that if you continued to go by ten with the amount of concentration then you would see that at 80 concentration 50% of the seeds would be germinated.
2. After getting the results from the two graphs the radicle data is supported by the germination data because both graphs had a significant drop as the amount of salt began to increase.
General Analysis and Conclusion:
Based on all the information that we had gathered from this lab my hypothesis was not correct. I had predicted that the more salt that was added into the water the more the radish seeds were going to germinate. But instead the results showed us that the less salt that was present in the petri dish the more the radish seeds would germinate. When there was 100% water and no salt the majority of the seeds were germinated, but when there was 100% salt and no water at all none of the seeds germinated. The results that we received in this lab are very similar to what the article says. This is true because in the article it says that in order for seeds to germinate they need water. The water that goes into the seeds will either directly germinate the seeds or it will react with the chemicals inside of the seed causing the seed to germinate. Most seeds need to receive a sustainable amount of water before they can germinate. After completing the lab and gathering all of the information I felt as though the lab would have been more accurate if we could have tried more various percentages of the salt in the water. But other than that overall the lab was very successful and most of the groups had all received around the same answers to the problem in this lab.
Citations:
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